Summary
- Description
- P[acman] BAC number CH321-73F24 has been chosen to target the gene Signal-transducer and activator of transcription protein at 92E. The LAP-TAG containing the GFP has been recombineered in 3' of the gene in frame. The engineered vector has then been inserted into the specific docking site with the help of Att sites. Recombinant individuals were screened based on mini-white presence. The line has been stabilized as homozygous for the transgene.
- Type
- insertion
- Purpose
- tagging
Modification method
- Nucleic acid delivery method
- stable transfection