ENCAB000AMI
Antibody against Homo sapiens USF2
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-862
- Lot ID
- J0908
- Characterized targets
- USF2 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Isotype
- IgG
- Antigen description
- Epitope mapping at the C-terminus of USF-2 of mouse origin.
- External resources
Characterizations
USF2 (Homo sapiens)
Method: immunoblot
not reviewed
- Caption
- Western blot protocol: Whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Results: Band of expected size visualized, representing strongest signal in the lane. Figure legend: IP-western with sc-862 in whole cell lysate (WCL) of SK-N-SH and GM12878; PM=protein marker. USF2 bands are indicated.
- Submitted by
- Richard Myers
- Lab
- Richard Myers, HAIB
- Grant
- U54HG004576
- Download
- human_USF2_C-20_validation_Myers.pdf