ENCAB000AHK

Antibody against Homo sapiens HDAC6

Homo sapiens
SKNSH, HepG2, GM12878, K562
characterized to standards with exemption
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-341A
Lot ID
1
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
bradley-bernstein:PchAb 290

Characterizations

HDAC6 (Homo sapiens)
K562HepG2GM12878SKNSH
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (7ug), GM12878 (7ug), SK-N-SH (6ug), were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a PVDF membrane using wet transfer. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a band of slightly above the expected size (~131kDa) was detected, probably due to post translational modifications.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
HDAC6 (Homo sapiens)
Method: immunoblot
not reviewed
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
HDAC6 (Homo sapiens)
Method: ChIP-string comparison
exempt from standards
Submitter comment
These characterizations were done in ENCODE2, before the current characterization methods.
Reviewer comment
These are being exempted as prior to standards. The associated primaries look good.
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad