ENCAB000AGY
Antibody against Homo sapiens GATA3
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-268
- Lot ID
- G0910
- Characterized targets
- GATA3 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Isotype
- IgG
- Antigen description
- Raised against human recombinant GATA-3.
- External resources
Characterizations
GATA3 (Homo sapiens)
Method: ChIP-seq comparison
not reviewed
- Caption
- GATA3 ChIP-seq peak comparison: GATA3 ChIP-seq was performed in MCF7 cells using two independent anti-GATA3 antibodies, sc-268 and sc-269. Peaks were called for both datasets and the overlap was analyzed. The figure shows that a large proportion of GATA3 peaks are shared between the two datasets.
- Submitted by
- Peggy Farnham
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
GATA3 (Homo sapiens)
Method: immunoblot
not reviewed
- Caption
- Western blot protocol: 40 ug of nuclear extract from MCF7 cells was loaded and separated with a Bio-Rad Mini-PROTEAN TGX SDS-PAGE system and transferred to a nitrocellulose membrane for Western blotting. Western blots were performed with primary GATA3 antibody at a concentration of 1ug/0.5mL in 5% milk-TBST. Results: Band of expected size visualized. Figure legend: Western with sc-268 in nuclear extract of MCF7 cells; PM=protein marker. GATA3 bands are indicated.
- Submitted by
- Peggy Farnham
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558