1. Antibodies
  2. Homo sapiens
  3. E2F6

ENCAB000AFW

Antibody against Homo sapiens E2F6

Homo sapiens
any cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-22823
Lot ID
D1206
Characterized targets
E2F6 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Antigen description
Epitope corresponding to a.a. 232-281 mapping at the C-terminus of E2F-6 of human origin

Characterizations

E2F6 (Homo sapiens)
Method: knockdown or knockout
Attachment PDF Icon
not reviewed
Caption
A. Antibody specificty was determined by Western blotting. HeLa(lane20,HepG2(lane3) and K562(lane4) nuclear extracts were preobed with E2F6 rabbit pAb. B. A plasmid that expresses shRNAs against E2F6 was stably introduced into Ntera2 cells and then ChIP experiments were prefomred in control and knockdown cells. the E2F6 signals were greatly reduced at all target genes but the enrichment of the same target genes by E2F1 or E2F4 was not reduced. C. Summary: The E2F6 antibody recongnized the right size band, enriches for a large number of known E2F target sites, and the enrichment is lost when the E2F6 mRNA is reduced.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford
E2F6 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
not reviewed
Caption
Western blot protocol: Whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Results: Band of expected size visualized, representing strongest signal in the lane. Figure legend: IP-western with sc-22823 in whole cell lysates (WCL) of K562 and HepG2; PM=protein marker. E2F6 band is indicated, as are heavy and light chains of IgG.
Submitted by
Richard Myers
Lab
Richard Myers, HAIB