ENCAB000AEW
Antibody against Homo sapiens CBX3
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-101004
- Lot ID
- G1410
- Characterized targets
- CBX3 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Isotype
- IgG
- Antigen description
- Raised against recombinant CBX3 of human origin.
- External resources
Characterizations
CBX3 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
not reviewed
- Caption
- As per ENCODE data standards, all SEQUEST results are attached (ENCODE_HAIB_CBX3_sc101004_12072011_MassSpec.pdf), including common contaminants. Target protein is listed as entry no. 10a in the ~23 kDa band with 100% probability. CBX5 and CBX1 were also identified, indicating cross-reactivity of the antibody with these proteins that are similar to CBX3.
- Submitted by
- Richard Myers
- Lab
- Richard Myers, HAIB
- Grant
- U54HG004576
CBX3 (Homo sapiens)
Method: immunoblot
not reviewed
- Caption
- Western blot protocol: Whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Results: Band of expected size visualized, representing strongest signal in the lane. Heavy and light chain bands are also visualized. The ~23 kDa band was tested by mass spec in Validation 2. Figure legend: IP-western with sc-101004 in whole cell lysate (WCL) of K562, GM12878, HepG2 and HeLa; PM=protein marker. CBX3 bands are indicated.
- Submitted by
- Richard Myers
- Lab
- Richard Myers, HAIB
- Grant
- U54HG004576