ENCAB000AEQ

Antibody against Homo sapiens FOS

Homo sapiens
MCF-7, IMR-90
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
K562, HeLa-S3, HepG2, A549, GM12878, heart
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-7202
Lot ID
K0810
Characterized targets
FOS (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
amino acids 210-335 mapping at the C-terminus of c-Fos of human origin.

Characterizations

FOS (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody sc-7202. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 62.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
FOS (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target FOS is represented by the attached position weight matrix (PWM) derived from ENCFF277MLU. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.828394776 Global enrichment Z-score: 12.06665449 Positional bias Z-score: 14.72184371 Peak rank bias Z-score: 7.637519152 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
FOS (Homo sapiens)
IMR-90
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: IMR90, using the antibody sc-7202. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 62.0
Reviewer comment
Runs a bit smaller than expected size, but is within allowed 20% deviation.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
FOS (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-7202. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 62.0
Reviewer comment
Runs a bit smaller than expected size, but is within allowed 20% deviation.
Submitted by
Minyi Shi
Lab
Michael Snyder, Stanford
FOS (Homo sapiens)
HepG2MCF-7IMR-90
Method: immunoblot
Attachment from submitter
not compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: HepG2, MCF7, IMR90 using the antibody sc-7202. Molecular Weight: 62.0
Submitter comment
HepG2 should be reviewed. Isoform 2 (Uniprot: [P01100-2]) is around ~28kD for the smaller band.
Reviewer comment
Banding pattern is inconsistent across all cell types, particularly near expected size.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
FOS (Homo sapiens)
Method: immunoprecipitation
not reviewed
Caption
Western blot analysis of nuclear extracts prepared from multiple human cells lines using the anti c-Fos (sc-7202) antibody detects a band corresponding to the expected molecular weight of human c-Fos (62KD).
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
FOS (Homo sapiens)
Method: motif enrichment
not reviewed
Caption
Calculations were done by Alan Boyle using a collection of known motifs. Table 1 shows the fold-enrichments and fraction of peaks which contain the motif. The motif which produced the largest value for each criterion is shown in Table 1. Note that while the maximally enriched motifs may differ from the motif with the highest enrichment p-values and the most represented motif, the motifs are highly similar (Figure 1) and thus all values are similar between motifs. Motifs were identified using a matching stringency corresponding to 4-6 (6mer). Peaks identified by IDR (1% cutoff) were used in the analysis and +/-50bp from peak centers were considered. Enrichments are for a given motif vs. a background consisting of +/- 50bp from the centers of all DnaseI hypersensitive peaks. Repeat mask/simple repeats from UCSC and all gencode v7 exons (including non-protein coding genes) were excluded from the analysis. Comparison to shuffle motifs were used to correct for compositional bias. Enrichment is the corrected # of motifs in ChIP peaks/corrected # of motifs in DNaseI peaks. The current ENCODE standard calls for >4-fold enrichment and >10% motif representation for this criteria to be used for validation. The 4 c-Fos datasets presented here exceed these thresholds and sc-7202 is considered validated.
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
FOS (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90heartA549
Method: immunoblot
Attachment from submitter
not compliant
Caption
Western blot analysis of nuclear extracts prepared from multiple human cells lines using the anti c-Fos (sc-7202) antibody detects a band corresponding to the expected molecular weight of human c-Fos (62KD).
Reviewer comment
Non-compliant lanes either are not within 20% of expected size, or does not correspond to >50% of all bands on gel
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford