ENCAB000AED
Antibody against Homo sapiens BATF
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-100974
- Lot ID
- D0808
- Characterized targets
- BATF (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Antigen description
- Raised against recombinant B-ATF of human origin.
- External resources
Characterizations
BATF (Homo sapiens)
Method: immunoblot
not reviewed
- Caption
- Western blot protocol: Whole cell lysates were immunoprecipitated using primary antibody (sc-100974), and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Results: Band of expected size visualized, representing strongest signal in the lane. Figure legend: IP-western with sc-100974 in WCL (whole cell lysate) of GM12878; PM=protein marker. BATF band is indicated, as are heavy and light chain of IgG.
- Submitted by
- Richard Myers
- Lab
- Richard Myers, HAIB
- Grant
- U54HG004576