RNA-seq of long RNAs (single-end, unstranded)

  • Status: active
Title
RNA-seq of long RNAs (single-end, unstranded)
Assays
RNA-seq, single cell isolation followed by RNA-seq
Description
Pipeline for single-ended unstranded data long RNA-seq data as developed by the ENCODE RNA Working Group. RNA libraries generated from mRNAs (poly-A(+)), rRNA-depleted total RNA, or poly-A(-) populations that are size-selected to be longer than approximately 200 bp may be run through this pipeline. More information regarding uniform pipeline restrictions, references used for this pipeline, and exogenous RNA spike-in controls are described in the pipelines overview document.
Lab
ENCODE Processing Pipeline

Pipeline schematic

Documents

pipeline protocol
Description excerpt: Long RNA-seq pipeline: Includes overview, references, and spike-ins…
Description
Long RNA-seq pipeline: Includes overview, references, and spike-ins information about the pipeline
Submitted by
Kathrina Onate
Lab
J. Michael Cherry, Stanford