Summary
- Description
- To generate v-Src:ER, DNA sequences encoding the Schmidt Ruppin A form of v-Src (a gift from Josh Kaplan) were fused to hbER and cloned into the pWZLblast3, pBabepuro3, or pLNCX retroviral expression vectors. In the case of pWZLblast3 (a gift from Jay Morgenstern), expression of a bicistronic mRNA from the murine leukemia virus long terminal repeat containing the encephalomyocarditis virus internal ribosome entry site permits the expression of both v-Src:ER and the blasticidin drug resistance gene - from PMID:9891045.
- Type
- insertion
- Purpose
- tagging
Modification method
- Nucleic acid delivery method
- stable transfection