Summary
- Description
- This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology and Genetics in Dresden, using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The MED-1::EGFP fusion protein is expressed in the correct med-1 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the MED-1 transcription factor.
- Type
- insertion
- Tags
- eGFP — C-terminal
- FLAG — C-terminal
- Purpose
- tagging
Modification method
- Nucleic acid delivery method
- bombardment