ENCGM515WNZ

Summary

Status
released
Description
This construct was made using the P[acman] Drosophila Melanogaster Library and contains a GFP (AV007) tag at the C-terminal of Dif, targeting these isoforms: Dif-RA, Dif-RB, Dif-RD. The 50bp preceding the tag are: TCCAGGACATTGAGGCAGAACTCAATAGCTTGGGAATTCAGCCATTCAAA
Type
insertion
Tags
  • eGFPC-terminal
  • 3xFLAGC-terminal
Purpose
tagging

Modification site

Modification method

Method
site-specific recombination
Treatments
  • Integrase (UniProtKB:Q9T221)