ENCGM325CMD

Summary

Status
released
Description
wgIs465 [nhr-179::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE MADE BY:DKV-RJT-PW RECEIVED:03-14-12 from Reinke V, modENCODE, Yale University, New Haven, CT
Type
insertion
Tags
  • eGFPC-terminal
Purpose
tagging

Modification site

Integration site
random

Modification method

Nucleic acid delivery method
bombardment