Summary for ENCSR369UFO

Summary

Status
released
Description
Functional characterization series for CRISPRi/dCas9-RYBP sparse-tiling proliferation screens at ~7500 cCREs around ~450 essential genes in K562
Donor diversity
single
Assay
proliferation CRISPR screen
Biosample summary
K562 cell line treated with Puromycin genetically modified (insertion) using transduction, genetically modified (CRISPRi) using CRISPR
Diseases
Not reported
Treatments
Puromycin
Elements references
Tiling modality
sparse peak

Attribution

ENCODE4 project
Lab
Will Greenleaf, Stanford
Award
UM1HG009436 (Will Greenleaf, Stanford)
Project
ENCODE
Aliases
will-greenleaf:K562_LargeScale_DHS_Growth_dCas9-RYBP
External resources
None submitted

Experiments in ENCSR369UFO

Experiments
2 experiments
Accession
Assay
Examined loci
Biosample summary
Lab
Status
Cart
ENCSR546ILAproliferation CRISPR screenHomo sapiens K562, 21 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (sgRNA)Will Greenleaf, Stanford
released
ENCSR398UPEproliferation CRISPR screenHomo sapiens K562, 7 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (sgRNA)Will Greenleaf, Stanford
released
Control experiments
1 control experiment
Accession
Control type
Biosample summary
Lab
Status
Cart
ENCSR016FPPcontrolWill Greenleaf, Stanford
released