Summary for ENCSR127CQV
Summary
- Status
- released
- Description
- Functional characterization series for dCas9-KRAB/CRISPRi proliferation screens at CTCF motifs in loop anchors
- Donor diversity
- single
- Assay
- proliferation CRISPR screen
- Biosample summary
- K562 cell line treated with Puromycin genetically modified (insertion) using transduction, genetically modified (CRISPRi) using CRISPR , for CTCF locus
- Diseases
- Not reported
- Treatments
- Puromycin
- Elements references
- Tiling modality
- sparse peak
Attribution
ENCODE4 project
- Lab
- Will Greenleaf, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- will-greenleaf:CRISPRi_CTCF_Motif_Growth
- External resources
- None submitted
Experiments in ENCSR127CQV
Experiments
2 experiments
Accession | Assay | Examined loci | Biosample summary | Lab | Status | Cart |
|---|---|---|---|---|---|---|
| ENCSR371TIR | proliferation CRISPR screen | Homo sapiens K562, 7 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for CTCF locus | Will Greenleaf, Stanford | released | ||
| ENCSR223KWN | proliferation CRISPR screen | Homo sapiens K562, 21 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for CTCF locus | Will Greenleaf, Stanford | released |
Control experiments
1 control experiment
Accession | Control type | Biosample summary | Lab | Status | Cart |
|---|---|---|---|---|---|
| ENCSR067VXW | control | Will Greenleaf, Stanford | released |