Functional Characterization Experiment summary for ENCSR897BXT

Summary

Status
released
Assay
MPRA (Control MPRA)
Control type
control
Biosample summary
Homo sapiens K562 genetically modified (insertion) using transduction
Biosample Type
cell line
Replication type
isogenic
Description
Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential WTC11 enhancers and promoters of all protein-coding genes.
Nucleic acid type
DNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Elements references
Elements mappings

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
Aliases
nadav-ahituv:joint_elements-DNA, nadav-ahituv:joint_k562_elements-DNA
Date released
December 21, 2023

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line genetically modified (insertion) using transductionENCBS958VMIENCGM344VZSENCLB880ZAK
21Homo sapiens K562 cell line genetically modified (insertion) using transductionENCBS519AACENCGM344VZSENCLB416ATA
31Homo sapiens K562 cell line genetically modified (insertion) using transductionENCBS821CGAENCGM344VZSENCLB830TOX