Functional Characterization Experiment summary for ENCSR897BXT
Summary
- Status
- released
- Assay
- MPRA (Control MPRA)
- Control type
- control
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential WTC11 enhancers and promoters of all protein-coding genes.
- Nucleic acid type
- DNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Platform
- Illumina NextSeq 500
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- Aliases
- nadav-ahituv:joint_elements-DNA, nadav-ahituv:joint_k562_elements-DNA
- Date released
- December 21, 2023
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
---|---|---|---|---|---|
1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction | ENCBS958VMI | ENCGM344VZS | ENCLB880ZAK |
2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction | ENCBS519AAC | ENCGM344VZS | ENCLB416ATA |
3 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction | ENCBS821CGA | ENCGM344VZS | ENCLB830TOX |