Functional Characterization Experiment summary for ENCSR837DCD
Summary
- Status
- released
- Assay
- MPRA (Control MPRA)
- Control type
- control
- Biosample summary
- Homo sapiens HepG2 genetically modified (insertion) using transduction
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential enhancers from HepG2, K562, and WTC11 cells, and promoters of protein-coding genes.
- Nucleic acid type
- DNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Platform
- Illumina NextSeq 500
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- Aliases
- nadav-ahituv:joint_hepg2-elements-DNA
- Date released
- December 21, 2023
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS085ALE | ENCGM344VZS | ENCLB198RAG |
| 3 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS757EJG | ENCGM344VZS | ENCLB390QPN |
| 2 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS351FUL | ENCGM344VZS | ENCLB990QQU |