Functional Characterization Experiment summary for ENCSR540PAH
doi:10.17989/ENCSR540PAH
Summary
- Status
- released
- Assay
- MPRA (Control MPRA)
- Control type
- control
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transient transfection for PKLR locus
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the PKLR promoter.
- Nucleic acid type
- DNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Platform
- Illumina NextSeq 500
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- References
- Aliases
- nadav-ahituv:PKLR-48-mpra-satMut-DNA
- Date released
- October 11, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transient transfection | ENCBS892VEN | ENCGM955MLH | ENCLB538EPQ |
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transient transfection | ENCBS449YMQ | ENCGM955MLH | ENCLB393CVT |
| 3 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transient transfection | ENCBS702DCE | ENCGM955MLH | ENCLB674EKZ |