Functional Characterization Experiment summary for ENCSR501FEU

doi:10.17989/ENCSR501FEU

Summary

Status
released
Assay
CRISPR screen (Control CRISPR screen)
Control type
control
Biosample summary
Homo sapiens K562 genetically modified (insertion) using transduction
Biosample Type
cell line
Tiling modality
sparse peak
Replication type
isogenic
Description
CRISPR-dCas9-based epigenomic regulatory element screening (CERES) technology to screen a subset of putative non-coding regulatory elements identified in human K562 leukemia cells from whole genome discovery screen (validation). This validation screen was performed in K562 leukemia cells
Nucleic acid type
DNA
Elements references

Attribution

ENCODE4 project
Lab
Tim Reddy, Duke
Award
UM1HG009428 (Tim Reddy, Duke)
Project
ENCODE
Aliases
charles-gersbach:encode4-ceres-wt-validation-screen-k562
Date submitted
August 31, 2021
Date released
October 20, 2022
Functional Characterization Series
ENCSR274OEB 
Submitter comment
Wild type

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
31Homo sapiens K562 cell line genetically modified (insertion) using transductionENCBS105LLMENCGM809XEKENCLB675LBI
41Homo sapiens K562 cell line genetically modified (insertion) using transductionENCBS301PKYENCGM809XEKENCLB339TRC
21Homo sapiens K562 cell line genetically modified (insertion) using transductionENCBS974GZBENCGM809XEKENCLB076DDH
11Homo sapiens K562 cell line genetically modified (insertion) using transductionENCBS152SVKENCGM809XEKENCLB296LMO