Functional Characterization Experiment summary for ENCSR360LZF

doi:10.17989/ENCSR360LZF

Summary

Status
released
Assay
MPRA (Control MPRA)
Control type
control
Biosample summary
Homo sapiens HepG2 genetically modified using transient transfection
Biosample Type
cell line
Replication type
isogenic
Description
Elements cloned into the pGL4 vector upstream of a minimal promoter. Test of the same set of elements in both the forward and reverse orientations in parallel.
Nucleic acid type
DNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Strand specificity
Strand-specific (unstranded)
Elements mapping
ENCSR200NQW

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
Aliases
nadav-ahituv:FwdRev-mpra-DNA
Date submitted
December 2, 2020
Date released
February 19, 2021

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
41HepG2 cell line genetically modified (insertion) using transient transfectionENCBS597FXXENCGM788EQEENCLB053EFM
11HepG2 cell line genetically modified (insertion) using transient transfectionENCBS352PXLENCGM788EQEENCLB376TET
21HepG2 cell line genetically modified (insertion) using transient transfectionENCBS497PYPENCGM788EQEENCLB505XOH
31HepG2 cell line genetically modified (insertion) using transient transfectionENCBS573RKNENCGM788EQEENCLB206HFS