1. Functional Characterization Experiments
  2. MPRA
  3. Homo sapiens
  4. HepG2

Functional Characterization Experiment summary for ENCSR331WRX

doi:10.17989/ENCSR331WRX

Summary

Status
released
Assay
MPRA (Control MPRA)
Control type
control
Biosample summary
Homo sapiens HepG2 genetically modified (insertion) using transduction
Biosample Type
cell line
Replication type
isogenic
Description
Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.
Nucleic acid type
DNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Elements references
Elements mappings

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
Aliases
nadav-ahituv:strands-mpra-DNA
Date released
August 17, 2022
ENCLB825LNI submitter comment
This library previously had strand_specificity of "strand-specific" but following an upgrade, strand_specificity no longer belongs on DNA libraries and has since been removed.
ENCLB623VAH submitter comment
This library previously had strand_specificity of "strand-specific" but following an upgrade, strand_specificity no longer belongs on DNA libraries and has since been removed.
ENCLB898UME submitter comment
This library previously had strand_specificity of "strand-specific" but following an upgrade, strand_specificity no longer belongs on DNA libraries and has since been removed.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens HepG2 cell line genetically modified (insertion) using transductionENCBS952IEQENCGM836MHVENCLB825LNI
11Homo sapiens HepG2 cell line genetically modified (insertion) using transductionENCBS972OVNENCGM836MHVENCLB623VAH
31Homo sapiens HepG2 cell line genetically modified (insertion) using transductionENCBS117QYDENCGM836MHVENCLB898UME

Files

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Output type
Replicates

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