Functional Characterization Experiment summary for ENCSR325SWE

doi:10.17989/ENCSR325SWE

Summary

Status
released
Assay
CRISPR screen (Control CRISPR screen)
Control type
control
Biosample summary
Homo sapiens PC-3 genetically modified using CRISPR (sgRNA), transduction 0 days after the sample was treated with 2 μg/mL puromycin for 2 days, 0.2 μg/mL puromycin for 2 days
Biosample Type
cell line
Perturbation type
interference
Replication type
isogenic
Description
Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
Treatments
2 μg/mL puromycin (CHEBI:17939) for 2 days, 0.2 μg/mL puromycin (CHEBI:17939) for 2 days
Nucleic acid type
RNA
Size range
710-720
Strand specificity
unstranded
Controls
Elements references

Attribution

ENCODE4 project
Lab
Bing Ren, UCSD
Award
UM1HG009402 (Yin Shen, UCSF)
Project
ENCODE
Aliases
bing-ren:BC19E_D0_PC3
Date submitted
January 31, 2020
Date released
August 6, 2020

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11PC-3 cell line genetically modified (insertion) using transduction, genetically modified (interference) using CRISPR (sgRNA) 0 days after the sample was treated with 2 μg/mL puromycin for 2 days, 0.2 μg/mL puromycin for 2 daysENCBS872YFFENCGM554RTS, ENCGM353IMQENCLB503CBC
21PC-3 cell line genetically modified (insertion) using transduction, genetically modified (interference) using CRISPR (sgRNA) 0 days after the sample was treated with 2 μg/mL puromycin for 2 days, 0.2 μg/mL puromycin for 2 daysENCBS841QGNENCGM554RTS, ENCGM353IMQENCLB864RSJ