Experiment summary for ENCSR995GRL
doi:10.17989/ENCSR995GRL
Summary
- Status
- released
- Assay
- RNA-seq (total RNA-seq)
- Biosample summary
- Homo sapiens activated T-cell female adult (21 years) treated with 50 U/mL Interleukin-2 for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours
- Biosample Type
- primary cell
- Replication type
- unreplicated
- Description
- CD3+_DONOR 4: 72 Hour T cell activation (CD3.CD28 beads) + 50U/ml IL-2
- Treatments
- 50 U/mL Interleukin-2 (UniProtKB:P60568) for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours
- Nucleic acid type
- RNA
- RNA integrity number
- 9
- Library starting quantity
- 330ng
- Average fragment size
- 281 [1-4]232 [1-2]279 [1-1]277 [1-3]
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR597UQL
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE4 project
- Lab
- Barbara Wold, Caltech
- Award
- UM1HG009443 (Barbara Wold, Caltech)
- Project
- ENCODE
- External resources
- Date released
- July 1, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 4 | Homo sapiens female adult (21 years) activated T-cell primary cell treated with 50 U/mL Interleukin-2 for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours | ENCBS257FNC | ENCLB363NWV |
| 1 | 2 | Homo sapiens female adult (21 years) activated T-cell primary cell treated with 50 U/mL Interleukin-2 for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours | ENCBS257FNC | ENCLB357ADU |
| 1 | 1 | Homo sapiens female adult (21 years) activated T-cell primary cell treated with 50 U/mL Interleukin-2 for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours | ENCBS257FNC | ENCLB008GSE |
| 1 | 3 | Homo sapiens female adult (21 years) activated T-cell primary cell treated with 50 U/mL Interleukin-2 for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours | ENCBS257FNC | ENCLB474RDC |