Experiment summary for ENCSR994CUJ

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP748 (wgIs748(ceh-6::TY1 EGFP 3xFLAG(P000007_A02_9H01);unc-119(+));unc-119 (ed3); VC2010) whole organism late embryo (3.3 hours) using egg bleaching genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing ceh-6-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:CEH-6_OP748_LE
Date submitted
May 30, 2018
Date released
June 12, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
21strain OP748 (wgIs748(ceh-6::TY1 EGFP 3xFLAG(P000007_A02_9H01);unc-119(+));unc-119 (ed3); VC2010) mixed sex late embryo (3.3 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-ceh-6ENCBS639UNGENCGM418EZGENCAB728YTOENCLB333TFR
11strain OP748 (wgIs748(ceh-6::TY1 EGFP 3xFLAG(P000007_A02_9H01);unc-119(+));unc-119 (ed3); VC2010) mixed sex late embryo (3.3 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-ceh-6ENCBS195ZBZENCGM418EZGENCAB728YTOENCLB935ORL