Experiment summary for ENCSR985TNB

Summary

Status
released
Assay
ChIP-seq
Target
eGFP-foxo
Biosample summary
Drosophila melanogaster MI00493 whole organism wandering third instar larva genetically modified using site-specific recombination
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing foxo-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:foxo-MIMIC-GFP
Date submitted
April 10, 2016
Date released
November 22, 2017

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
21strain foxo-MiMIC-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-foxoENCBS750VJXENCGM493WFPENCAB728YTOENCLB885VNE
11strain foxo-MiMIC-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-foxoENCBS585WXEENCGM493WFPENCAB728YTOENCLB898KEK
31strain foxo-MiMIC-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-foxoENCBS997LAMENCGM493WFPENCAB728YTOENCLB503MAL