Experiment summary for ENCSR969MNX

doi:10.17989/ENCSR969MNX

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
lsl-1
Biosample summary
Caenorhabditis elegans strain OP720 (unc-119(tm4063); wgIs720 [lsl-1::TY1::EGFP::3xFLAG + unc-119(+)]) whole organism young adult (69 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans lsl-1
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing lsl-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:LSL-1_OP720_yAd
Date submitted
February 26, 2018
Date released
March 1, 2018

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Caenorhabditis elegans strain OP720 (unc-119(tm4063); wgIs720 [lsl-1::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex young adult (69 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans lsl-1ENCBS106TAZENCGM879VHAENCAB728YTOENCLB200ESK
21Caenorhabditis elegans strain OP720 (unc-119(tm4063); wgIs720 [lsl-1::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex young adult (69 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans lsl-1ENCBS551YSAENCGM879VHAENCAB728YTOENCLB464JLY