Experiment summary for ENCSR961YAG

doi:10.17989/ENCSR961YAG

Superseded by ENCSR264MSX

Summary

Status
archived
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
EIF4A3
Biosample summary
Homo sapiens K562 expressing RNAi targeting H. sapiens EIF4A3
Biosample Type
cell line
Replication type
isogenic
Description
shRNA knockdown against EIF4A3 in K562 cells followed by RNA-seq. (EIF4A3-LV08)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.7 [1-1]9.2 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:EIF4A3-LV08
Date submitted
September 15, 2014
Date released
October 16, 2014
Gene Silencing Series
ENCSR733XTT 
Submitter comment
As of November 2022, this dataset is superseded by ENCSR264MSX, which has a better knockdown rate.
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv1Search for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line expressing RNAi targeting H. sapiens EIF4A3ENCBS038TWGENCGM966QHBENCLB179JOW
21Homo sapiens K562 cell line expressing RNAi targeting H. sapiens EIF4A3ENCBS119EVPENCGM966QHBENCLB507CNH