Experiment summary for ENCSR954HAY

doi:10.17989/ENCSR954HAY

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
SART3
Biosample summary
Homo sapiens K562 expressing RNAi targeting H. sapiens SART3
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on K562 cells treated with an shRNA knockdown against SART3. (SART3_BGKLV24_B)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
8.6 [1-1]9.4 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:SART3_BGKLV24_B
Date submitted
December 7, 2015
Date released
January 12, 2016
Gene Silencing Series
ENCSR396HJG 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line expressing RNAi targeting H. sapiens SART3ENCBS166SNLENCGM276YDNENCLB954HKX
21Homo sapiens K562 cell line expressing RNAi targeting H. sapiens SART3ENCBS415MYOENCGM276YDNENCLB677NTM