Experiment summary for ENCSR936TED

doi:10.17989/ENCSR936TED

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
POLR2G
Biosample summary
Homo sapiens K562 expressing RNAi targeting H. sapiens POLR2G
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on K562 cells treated with an shRNA knockdown against POLR2G. (K-POLR2G-30B)
Nucleic acid type
RNA
RNA integrity number
9.6 [2-1]9.7 [1-1]
Depleted in
rRNA
Size range
>200
Extraction method
Trizol
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:K-POLR2G-30B
Date submitted
May 14, 2014
Date released
June 30, 2014
Gene Silencing Series
ENCSR100JYY 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens K562 cell line expressing RNAi targeting H. sapiens POLR2GENCBS023YVHENCGM906MYIENCLB477GKN
11Homo sapiens K562 cell line expressing RNAi targeting H. sapiens POLR2GENCBS865CZCENCGM906MYIENCLB575IMJ