Experiment summary for ENCSR929PXS

doi:10.17989/ENCSR929PXS

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
CEBPZ
Biosample summary
Homo sapiens HepG2 expressing RNAi targeting H. sapiens CEBPZ
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on HepG2 cells treated with an shRNA knockdown against CEBPZ. (CEBPZ_BGHLV30)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
10
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:CEBPZ_BGHLV30
Date submitted
March 4, 2016
Date released
March 16, 2016
Gene Silencing Series
ENCSR301KZX 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens CEBPZENCBS236NEPENCGM586EWRENCLB644YDC
11Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens CEBPZENCBS686ZOGENCGM586EWRENCLB516RRN