1. Experiments
  2. ChIP-seq
  3. Caenorhabditis elegans
  4. whole organism

Experiment summary for ENCSR891WMQ

doi:10.17989/ENCSR891WMQ

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
ceh-90
Biosample summary
Caenorhabditis elegans strain OP210 (unc-119(ed3) III; wgIs210) whole organism hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans ceh-90
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing CEH-90-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:CEH-90-OP210-L1
Date submitted
July 29, 2015
Date released
May 10, 2016
Submitter comment
ENCFF459SUT, ENCFF082HRL, ENCFF555KSQ, ENCFF341JWT were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Caenorhabditis elegans strain OP210 (unc-119(ed3) III; wgIs210) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans ceh-90ENCBS570XVFENCGM821OZZENCAB728YTOENCLB746ZMO
21Caenorhabditis elegans strain OP210 (unc-119(ed3) III; wgIs210) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans ceh-90ENCBS135PPMENCGM821OZZENCAB728YTOENCLB236VZM

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