Experiment summary for ENCSR880RBB

doi:10.17989/ENCSR880RBB

Summary

Status
revoked
Assay
ChIP-seq (TF ChIP-seq)
Target
pop-1
Biosample summary
Caenorhabditis elegans strain OP560 (unc-119(tm4063) III; wgIs560) whole organism early embryo 2.5 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans pop-1
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing POP-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:POP-1-OP560-EE
Date submitted
October 4, 2016
Date released
October 27, 2016
Submitter comment
The dataset was revoked because the lab indicated that the strain was found not to rescue mutants

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Caenorhabditis elegans strain OP560 (unc-119(tm4063) III; wgIs560) mixed sex early embryo 2.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans pop-1ENCBS449IVXENCGM003OCRENCAB728YTOENCLB166OLT
11Caenorhabditis elegans strain OP560 (unc-119(tm4063) III; wgIs560) mixed sex early embryo 2.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans pop-1ENCBS263WKKENCGM003OCRENCAB728YTOENCLB235MRW