Experiment summary for ENCSR880RBB

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP560 (unc-119(tm4063) III; wgIs560) whole organism early embryo 2.5 hours post synchronization using egg bleaching genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing POP-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation method
sonication (generic microtip)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:POP-1-OP560-EE
Date submitted
October 4, 2016
Date released
October 27, 2016
Submitter comment
ENCFF607FZP, ENCFF201FWX were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
21strain OP560 (unc-119(tm4063) III; wgIs560) mixed sex early embryo 2.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-pop-1ENCBS449IVXENCGM003OCRENCAB728YTOENCLB166OLT
11strain OP560 (unc-119(tm4063) III; wgIs560) mixed sex early embryo 2.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-pop-1ENCBS263WKKENCGM003OCRENCAB728YTOENCLB235MRW