1. Experiments
  2. shRNA knockdown followed by RNA-seq
  3. Drosophila melanogaster
  4. whole organism

Experiment summary for ENCSR877VRF

doi:10.17989/ENCSR877VRF

Alternate accession: ENCSR629YPW

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
bsh
Biosample summary
Drosophila melanogaster strain bsh_shRNA x da-Gal4 whole organism embryo 10-12 hours post synchronization at egg laying stage expressing RNAi targeting D. melanogaster bsh
Biosample Type
whole organisms
Replication type
isogenic
Nucleic acid type
polyadenylated mRNA
Size range
350-600
Extraction method
Trizol
Fragmentation methods
shearing (Covaris generic)
Strand specificity
unstranded
Controls

Attribution

modERN project
Lab
Susan Celniker, LBNL
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
susan-celniker:bsh_10-12
Date submitted
March 14, 2019
Date released
April 25, 2019
Submitter comment
Processed data is currently not available for these experiments, but will be added in the future.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Drosophila melanogaster strain bsh_shRNA x da-Gal4 embryo 10-12 hours post synchronization at egg laying stage whole organism expressing RNAi targeting D. melanogaster bshENCBS798KVUENCGM027YSKENCLB426PIV
21Drosophila melanogaster strain bsh_shRNA x da-Gal4 embryo 10-12 hours post synchronization at egg laying stage whole organism expressing RNAi targeting D. melanogaster bshENCBS438XTAENCGM027YSKENCLB893KEE

Files

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