Experiment summary for ENCSR875XJL

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
athp-1
Biosample summary
Caenorhabditis elegans strain OP685 (unc-119(tm4063) III; wgIs685) whole organism young adult 64 hours post synchronization at L1 larva starved after bleaching stage genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing ATHP-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:ATHP-1-OP685-yAd
Date submitted
October 5, 2017
Date released
November 6, 2017

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21strain OP685 (unc-119(tm4063) III; wgIs685) mixed sex young adult 64 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting athp-1ENCBS271CPRENCGM381EXNENCAB728YTOENCLB121MXQ
11strain OP685 (unc-119(tm4063) III; wgIs685) mixed sex young adult 64 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting athp-1ENCBS241THIENCGM381EXNENCAB728YTOENCLB991ZBY