Experiment summary for ENCSR828TEI
doi:10.17989/ENCSR828TEI
Summary
- Status
- released
- Assay
- RNA-seq (total RNA-seq)
- Biosample summary
- Homo sapiens myotube originated from skeletal muscle myoblast
- Biosample Type
- in vitro differentiated cells
- Replication type
- unreplicated
- Description
- The libraries contained in this experiment come from independent growths of primary cell line HSMM that has been differentiated into myotubes. They are stranded PE101 Illumina Hi-Seq RNA-Seq libraries from rRNA-depleted Total RNA > 200 nucleotides in size.
- Nucleic acid type
- RNA
- Depleted in
- rRNA
- Size range
- >200
- Fragmentation methods
- shearing (Covaris generic)
- Size selection method
- other
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR156CIL
- Platform
- Illumina HiSeq 2000
Attribution
ENCODE3 project
- Lab
- Thomas Gingeras, CSHL
- Award
- U54HG007004 (Thomas Gingeras, CSHL)
- Project
- ENCODE
- External resources
- References
- Date submitted
- October 7, 2014
- Date released
- November 20, 2014
- Reference Epigenome
- ENCSR207AHT
- Submitter comment
- In December 2018, the replication structure for this experiment was correct to 2 technical replicates, whereas previously, it was modeled as 2 biological replicates.
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 2 | Homo sapiens myotube in vitro differentiated cells originated from skeletal muscle myoblast | ENCBS526ENC | ENCLB796YUI |
| 1 | 1 | Homo sapiens myotube in vitro differentiated cells originated from skeletal muscle myoblast | ENCBS526ENC | ENCLB255LXZ |