Experiment summary for ENCSR824CLH

doi:10.17989/ENCSR824CLH

Summary

Status
released
Assay
small RNA-seq
Biosample summary
Drosophila melanogaster Kc167 nuclear fraction
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on rRNA-depleted total RNA in Drosophila cell line Kc167 (nuclear fraction), < 200 nucleotides in size that was CIP and TAP-treated prior to cloning.
Treatments
Antarctic Phosphatase for 30 minutes, Tobacco Acid Pyrophosphatase for 1 hour
Nucleic acid type
RNA
Depleted in
rRNA
Size range
<200
Extraction method
Trizol
Strand specificity
Strand-specific

Attribution

modENCODE Project
Lab
Thomas Gingeras, CSHL
Award
U01HG004271 (Susan Celniker, LBNL)
Project
modENCODE
Aliases
modencode:4211, susan-celniker:E-BS595N-BS596N_CIPTAP
Date submitted
March 31, 2014
Date released
March 31, 2014

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Drosophila melanogaster Kc167 cell line nuclear fractionENCBS780AESENCLB711FCO
21Drosophila melanogaster Kc167 cell line nuclear fractionENCBS851EXMENCLB144ICL