Experiment summary for ENCSR756TPG

doi:10.17989/ENCSR756TPG

Summary

Status
released
Assay
PRO-cap
Biosample summary
Homo sapiens K562
Biosample Type
cell line
Replication type
isogenic
Description
CoPRO experiment. A run-on assay was performed on captured nascent RNA with cap state selection. In this experiment, both capped and uncapped nascent RNA was selected and no PCR amplification was done.
Nucleic acid type
RNA
Average fragment size
47 [2-1]46 [2-2]47 [1-1]71 [1-2]
Size selection method
Streptavidin magnetic beads
Library construction method
PCR-free, capped RNA enrichment, uncapped RNA enrichment
Strand specificity
Strand-specific (reverse)

Attribution

ENCODE4 project
Lab
John Lis, Cornell
Award
UM1HG009393 (John Lis, Cornell)
Project
ENCODE
Aliases
john-lis:copro-experiment-CappedAndUncapped-PCRFree
Date submitted
November 19, 2021
Date released
December 14, 2021

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
21Homo sapiens K562 cell lineENCBS059DTNENCLB858GXI
22Homo sapiens K562 cell lineENCBS059DTNENCLB733QLQ
11Homo sapiens K562 cell lineENCBS223MNJENCLB570VMP
12Homo sapiens K562 cell lineENCBS223MNJENCLB390YOT