Experiment summary for ENCSR735PMJ
doi:10.17989/ENCSR735PMJ
Summary
- Status
- released
- Assay
- whole-genome shotgun bisulfite sequencing (WGBS)
- Biosample summary
- Mus musculus strain B6NCrl embryonic facial prominence tissue embryo (15.5 days)
- Biosample Type
- tissue
- Replication type
- isogenic
- Description
- Whole genome bisulfite-seq on embryonic 15.5 day mouse facial prominence
- Nucleic acid type
- DNA
- Size range
- 180-500
- Lysis method
- QIAGEN DNeasy Blood & Tissue Kit
- Fragmentation methods
- shearing (Covaris S2)
- Size selection method
- Sera-mag SpeedBeads
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Joe Ecker, Salk
- Award
- U54HG006997 (Bing Ren, UCSD)
- Project
- ENCODE
- External resources
- Aliases
- joe-ecker:E15_5_CF
- Date submitted
- April 28, 2015
- Date released
- June 23, 2015
- Reference Epigenome
- ENCSR899FPM
- Organism Development Series
- ENCSR008CAN
- Submitter comment
- Fastq files ENCFF543WUZ, ENCFF479IMU, ENCFF718YVL, and ENCFF327DSW contain reads of mixed lengths. Read lengths followed by respective number of reads: ENCFF543WUZ (117, 166958405), (125, 201057911), (145, 186298216); ENCFF479IMU (117, 167648028), (125, 197789140), (145, 168337772); ENCFF718YVL (117, 181829043), (125, 199420617), (130, 193006373); and ENCFF327DSW (117, 187732083), (125, 198645118), (130, 181651156).
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) embryonic facial prominence tissue | ENCBS543BPM | ENCLB635NUD |
| 2 | 1 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) embryonic facial prominence tissue | ENCBS900XQR | ENCLB773WZA |
| 1 | 2 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) embryonic facial prominence tissue | ENCBS543BPM | ENCLB148FSK |
| 2 | 2 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) embryonic facial prominence tissue | ENCBS900XQR | ENCLB174ICU |