Experiment summary for ENCSR693VPE
doi:10.17989/ENCSR693VPE
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- unc-86
- Biosample summary
- Caenorhabditis elegans strain OP476 (unc-119(tm4063) III; wgIs476) whole organism hermaphrodite late embryo 3.5 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans unc-86
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing UNC-86-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:UNC-86-OP476-LE
- Date submitted
- May 9, 2016
- Date released
- September 29, 2016
- Submitter comment
- ENCFF048WRE, ENCFF419REI, ENCFF629VSP, ENCFF568VRT were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
2 | 1 | Caenorhabditis elegans strain OP476 (unc-119(tm4063) III; wgIs476) hermaphrodite late embryo 3.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans unc-86 | ENCBS384PDK | ENCGM185NSY | ENCAB728YTO | ENCLB712WLP |
1 | 1 | Caenorhabditis elegans strain OP476 (unc-119(tm4063) III; wgIs476) hermaphrodite late embryo 3.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans unc-86 | ENCBS582ABT | ENCGM185NSY | ENCAB728YTO | ENCLB657OCR |