Experiment summary for ENCSR693MZJ

doi:10.17989/ENCSR693MZJ

Summary

Status
archived
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
HNRNPF
Biosample summary
Homo sapiens HepG2 expressing RNAi targeting H. sapiens HNRNPF
Biosample Type
cell line
Replication type
isogenic
Description
shRNA knockdown against HNRNPF in HepG2 cells followed by RNA-seq. (HNRNPF-BGHLV20)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
10 [1-1]9.9 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:HNRNPF-BGHLV20
Date submitted
August 13, 2015
Date released
August 18, 2015
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens HNRNPFENCBS399GAFENCGM202DGRENCLB524NQG
21Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens HNRNPFENCBS783YSPENCGM202DGRENCLB426VJX