Experiment summary for ENCSR684PHU

  • Status: released

Summary

Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP676 (unc-119(tm4063); wgIs676 [ztf-22::TY1::EGFP::3xFLAG + unc-119(+)]) whole organisms young adult (68 hours) at L1 larva starved after bleaching stage genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing -eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:ZTF-22_OP676_yAd
Date released
March 1, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
21strain OP676 (unc-119(tm4063); wgIs676 [ztf-22::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex young adult (68 hours) at L1 larva starved after bleaching stage whole organisms genetically modified (insertion) using bombardment targeting eGFP-ztf-22ENCBS957NNTENCGM207CCZENCAB728YTOENCLB096QVP
11strain OP676 (unc-119(tm4063); wgIs676 [ztf-22::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex young adult (68 hours) at L1 larva starved after bleaching stage whole organisms genetically modified (insertion) using bombardment targeting eGFP-ztf-22ENCBS933COAENCGM207CCZENCAB728YTOENCLB164SHB