Experiment summary for ENCSR680ZDH

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
pag-3
Biosample summary
Caenorhabditis elegans strain OP154 (unc-119(ed3) III; wgIs154) whole organism hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing PAG-3-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
E-Gel
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:PAG-3-OP154-L1
Date submitted
July 30, 2015
Date released
November 22, 2017
Submitter comment
This experiment was completed during modENCODE and has lower read depth than the current modERN standard.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21strain OP154 (unc-119(ed3) III; wgIs154) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting pag-3ENCBS297XRUENCGM183DVCENCAB435FBQENCLB868XGH
11strain OP154 (unc-119(ed3) III; wgIs154) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting pag-3ENCBS546NCPENCGM183DVCENCAB435FBQENCLB633TLD