Experiment summary for ENCSR680ZDH
doi:10.17989/ENCSR680ZDH
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- pag-3
- Biosample summary
- Caenorhabditis elegans strain OP154 (unc-119(ed3) III; wgIs154) whole organism hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans pag-3
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing PAG-3-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- E-Gel
- Platform
- Illumina Genome Analyzer IIx
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:PAG-3-OP154-L1
- Date submitted
- July 30, 2015
- Date released
- November 22, 2017
- Submitter comment
- This experiment was completed during modENCODE and has lower read depth than the current modERN standard.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
2 | 1 | Caenorhabditis elegans strain OP154 (unc-119(ed3) III; wgIs154) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans pag-3 | ENCBS297XRU | ENCGM183DVC | ENCAB435FBQ | ENCLB868XGH |
1 | 1 | Caenorhabditis elegans strain OP154 (unc-119(ed3) III; wgIs154) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans pag-3 | ENCBS546NCP | ENCGM183DVC | ENCAB435FBQ | ENCLB633TLD |