Experiment summary for ENCSR647NYX

doi:10.17989/ENCSR647NYX

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
NONO
Biosample summary
Homo sapiens HepG2 expressing RNAi targeting H. sapiens NONO
Biosample Type
cell line
Replication type
isogenic
Description
shRNA knockdown against NONO in HepG2 cells followed by RNA-seq. (NONO_BGHLV17)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.7 [1-1]9.6 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:NONO_BGHLV17
Date submitted
December 13, 2014
Date released
December 17, 2014
Gene Silencing Series
ENCSR905WEG 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens NONOENCBS282XVKENCGM403ZIXENCLB693YJG
21Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens NONOENCBS431NPJENCGM403ZIXENCLB920YRO