Experiment summary for ENCSR646OPC

doi:10.17989/ENCSR646OPC

Summary

Status
released
Assay
ChIP-seq (Histone ChIP-seq)
Target
H3K4me3
Biosample summary
Homo sapiens A549 treated with 100 nM dexamethasone (agonist) for 6 hours
Biosample Type
cell line
Replication type
isogenic
Description
H3K4me3 ChIP-seq on A549 cell line treated with 100 nM dexamethasone for 6 hours.
Treatments
100 nM dexamethasone (CHEBI:41879) for 6 hours
Nucleic acid type
DNA
Library starting quantity
7ng
Size range
>200
Fragmentation methods
sonication (Bioruptor Twin)
Size selection method
dual SPRI
Controls

Attribution

Genomics of Gene Regulation Project
Lab
Tim Reddy, Duke
Award
U01HG007900 (Tim Reddy, Duke)
Project
GGR
External resources
Aliases
tim-reddy:H3K4me3.t6
Date submitted
May 30, 2016
Date released
July 21, 2016
Treatment Time Series
ENCSR342NKR 

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Antibody
Library
21Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 6 hoursENCBS658RMMENCAB388GOHENCLB941DDD
11Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 6 hoursENCBS370PMJENCAB388GOHENCLB953PEV
31Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 6 hoursENCBS859WOFENCAB388GOHENCLB340TDE