Experiment summary for ENCSR637NLF
doi:10.17989/ENCSR637NLF
Summary
- Status
- archived
- Assay
- single-cell RNA sequencing assay (scRNA-seq)
- Control type
- control
- Biosample summary
- Mus musculus strain B6NCrl Purkinje cell male adult (8 months)
- Biosample Type
- primary cell
- Replication type
- unreplicated
- Description
- RNA-seq on single cell equivalents of 20 excised and homogenized Purkinje cells from an 8 month old male wild type C57Bl6 mouse
- Nucleic acid type
- RNA
- Library starting quantity
- 1cell-equivalent
- Size range
- >200
- Fragmentation methods
- chemical (Nextera tagmentation)
- Size selection method
- SPRI beads
- Strand specificity
- unstranded
- Spike-ins datasets
- ENCSR013YHQ, ENCSR133ALU
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Barbara Wold, Caltech
- Award
- U54HG006998 (Richard Myers, HAIB)
- Project
- ENCODE
- External resources
- Date submitted
- September 24, 2015
- Date released
- October 13, 2015
- Submitter comment
- Estimates of technical measurement error from low input mass RNA-seq libraries.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 6 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS620SZZ | ENCLB950QWJ |
| 1 | 1 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS459UCD | ENCLB435TVJ |
| 1 | 10 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS746KRI | ENCLB384GBY |
| 1 | 11 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS323MIZ | ENCLB030QYZ |
| 1 | 14 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS451ZMX | ENCLB807MQE |
| 1 | 15 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS610UUG | ENCLB462UWC |
| 1 | 2 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS107VUQ | ENCLB337IRK |
| 1 | 7 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS659MMZ | ENCLB219UWV |
| 1 | 12 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS456JNY | ENCLB721WMG |
| 1 | 16 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS716XAT | ENCLB296VEF |
| 1 | 5 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS721LLV | ENCLB577KCS |
| 1 | 9 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS281KUY | ENCLB430RUN |
| 1 | 3 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS881LWZ | ENCLB863GTU |
| 1 | 4 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS074RTA | ENCLB033OYZ |
| 1 | 13 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS825HER | ENCLB359XUW |
| 1 | 8 | Mus musculus strain B6NCrl male adult (8 months) Purkinje primary cell | ENCBS088CZP | ENCLB196QXY |