Experiment summary for ENCSR631RFX

doi:10.17989/ENCSR631RFX

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
MTPAP
Biosample summary
Homo sapiens K562 expressing RNAi targeting H. sapiens MTPAP
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on K562 cells treated with an shRNA knockdown against MTPAP. (MTPAP_BGKLV34)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
10
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR358OMW
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:MTPAP_BGKLV34
Date submitted
September 24, 2016
Date released
September 29, 2016
Gene Silencing Series
ENCSR564CIP 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens K562 cell line expressing RNAi targeting H. sapiens MTPAPENCBS966RJIENCGM665SEYENCLB956ZHW
11Homo sapiens K562 cell line expressing RNAi targeting H. sapiens MTPAPENCBS579ZTIENCGM665SEYENCLB988URW