Experiment summary for ENCSR622MCX

doi:10.17989/ENCSR622MCX

Summary

Status
archived
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
U2AF2
Biosample summary
Homo sapiens HepG2 expressing RNAi targeting H. sapiens U2AF2
Biosample Type
cell line
Replication type
isogenic
Description
shRNA knockdown against U2AF2 in HepG2 cells followed by RNA-seq. (U2AF2 BGHLV14)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.3 [2-1]9.5 [1-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:U2AF2_BGHLV14
Date submitted
November 4, 2014
Date released
November 20, 2014
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv1Search for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens U2AF2ENCBS727GLBENCGM741JYCENCLB948TVP
11Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens U2AF2ENCBS970EPGENCGM741JYCENCLB236VAS