Experiment summary for ENCSR590GUK

Summary

Status
released
Assay
RNA-seq (polyA RNA-seq)
Biosample summary
Mus musculus C57BL/6 bone marrow-derived macrophage originated from bone marrow treated with 100 ng/mL lipoprotein A for 12 hours, 100 ng/mL lipoprotein A for 480 minutes
Biosample Type
primary cell
Replication type
isogenic
Description
mRNA-seq on whole cell extract RNA which is part of BMDM cell pre-treated with 100 ng/ml Lipid A 12 hours, washed out for 12 hours, and stimulated with 100 ng/ml Lipid A for 480 minutes.
Treatments
100 ng/mL lipoprotein A for 12 hour, 100 ng/mL lipoprotein A for 480 minute
Nucleic acid type
polyadenylated mRNA
Library starting quantity
1µg
Size range
250-400
Extraction method
see document
Fragmentation method
see document
Size selection method
SPRI beads
Strand specificity
Non-strand-specific

Attribution

Genomics of Gene Regulation Project
Lab
Alexander Hoffmann, UCLA
Award
U01HG007912 (Alexander Hoffmann, UCLA)
Project
GGR
Aliases
alexander-hoffmann:PolyA.LPA.480.exp
Date submitted
April 5, 2018
Date released
November 20, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleLibrary
11strain C57BL/6J bone marrow-derived macrophage primary cell originated from bone marrow treated with 100 ng/mL lipoprotein A for 12 hours, 100 ng/mL lipoprotein A for 480 minutesENCBS447UBWENCLB397IJD
21strain C57BL/6J bone marrow-derived macrophage primary cell originated from bone marrow treated with 100 ng/mL lipoprotein A for 12 hours, 100 ng/mL lipoprotein A for 480 minutesENCBS968XQGENCLB107ECQ
31strain C57BL/6J bone marrow-derived macrophage primary cell originated from bone marrow treated with 100 ng/mL lipoprotein A for 12 hours, 100 ng/mL lipoprotein A for 480 minutesENCBS863XKBENCLB635EZY