Experiment summary for ENCSR583EFI

Summary

Status
released
Assay
ChIP-seq
Target
eGFP-ss
Biosample summary
Drosophila melanogaster VK00037 whole organism prepupa (24 hours) genetically modified using site-specific recombination
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing ss-RA-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:ss-RA-GFP
Date submitted
September 11, 2017
Date released
February 1, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
11strain ss-GFP mixed sex prepupa (24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-ssENCBS386TZQENCGM108KYLENCAB728YTOENCLB595BJJ
21strain ss-GFP mixed sex prepupa (24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-ssENCBS362LUTENCGM108KYLENCAB728YTOENCLB163SLY