Experiment summary for ENCSR549VKC

doi:10.17989/ENCSR549VKC

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
tbx-8
Biosample summary
Caenorhabditis elegans strain RW10470 (st10470 [tbx-8::TY1::EGFP::3xFLAG; unc-119(+)]; unc-119(ed3)) whole organism mixed stage (embryo) (2.5 hours) using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans tbx-8
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing tbx-8-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:TBX-8_RW10470_EE
Date submitted
February 26, 2018
Date released
March 1, 2018

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Caenorhabditis elegans strain RW10470 (st10470 [tbx-8::TY1::EGFP::3xFLAG; unc-119(+)]; unc-119(ed3)) mixed sex mixed stage (embryo) (2.5 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans tbx-8ENCBS464TCXENCGM068KVMENCAB728YTOENCLB974CDJ
21Caenorhabditis elegans strain RW10470 (st10470 [tbx-8::TY1::EGFP::3xFLAG; unc-119(+)]; unc-119(ed3)) mixed sex mixed stage (embryo) (2.5 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans tbx-8ENCBS307YXHENCGM068KVMENCAB728YTOENCLB598WSF