Experiment summary for ENCSR549VKC
doi:10.17989/ENCSR549VKC
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- tbx-8
- Biosample summary
- Caenorhabditis elegans strain RW10470 (st10470 [tbx-8::TY1::EGFP::3xFLAG; unc-119(+)]; unc-119(ed3)) whole organism mixed stage (embryo) (2.5 hours) using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans tbx-8
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing tbx-8-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:TBX-8_RW10470_EE
- Date submitted
- February 26, 2018
- Date released
- March 1, 2018
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
1 | 1 | Caenorhabditis elegans strain RW10470 (st10470 [tbx-8::TY1::EGFP::3xFLAG; unc-119(+)]; unc-119(ed3)) mixed sex mixed stage (embryo) (2.5 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans tbx-8 | ENCBS464TCX | ENCGM068KVM | ENCAB728YTO | ENCLB974CDJ |
2 | 1 | Caenorhabditis elegans strain RW10470 (st10470 [tbx-8::TY1::EGFP::3xFLAG; unc-119(+)]; unc-119(ed3)) mixed sex mixed stage (embryo) (2.5 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans tbx-8 | ENCBS307YXH | ENCGM068KVM | ENCAB728YTO | ENCLB598WSF |